Extended Data Fig. 5: The lactate level in the somata was lower than that in the terminals.
a, The ratio between mTFP and Venus fluorescence (at 430 nm excitation) was measured at 0.001, 0.01, 0.1, 1 and 10 mM lactate in an ex vivo brain slice (n = 28 from 3 mice). b, Images of mTFP (cyan) and Venus (magenta) fluorescence and the corresponding mTFP/Venus ratio (colour-coded, scale on the right) in the SSp brain slices. Synaptophysin-mCherry was expressed to locate nerve terminals (red). c, Quantification of the mTFP/Venus ratio in the somata (n = 58 from 3 mice) and nerve terminals (n = 88 from 3 mice), p < 0.0001. For the boxplots, the centre line indicates the median, the box limits indicate the first and third quartiles, and the whiskers indicate the data range. Scale bars, 50 μm. Data are presented as mean ± SEM and ***p < 0.001. Student’s two-tailed unpaired t-test (c).
