Extended Data Fig. 6: PKM2 express higher in isolated neuronal somata than synaptosomes.
a, Capillary western blot analysis of PKM1 and PKM2 in primary neurons expressed with 3×flag-tagged PKM1 or PKM2. The samples are normalised to the total protein indicated by blue circles (n = 3 independent experiments). b, Capillary western blot analysis of PKM1 and PKM2 in the somata (S) and nerve terminals (T). The samples are normalised to the total protein indicated by blue circles. c, Statistical analysis of protein band intensity normalised to the total protein in somata and nerve terminals (n = 3 biological replicates). d, Capillary western blot analysis PKM2 in the cytoplasm and nucleus of isolated neuronal somata. The samples are normalised to the total protein indicated by blue circles. e, Statistical analysis of protein band intensity normalised to the total protein in the cytoplasm and nucleus (n = 3 biological replicates, p < 0.0001). f, The PKM2 dimer and tetramer formation in isolated neuronal somata was analysed by blue native page. g, Statistical analysis of the PKM2 dimer and tetramer band intensity (n = 3 biological replicates, p = 0.0011). Data are presented as mean ± SEM and **p < 0.01 and ***p < 0.001. Two-way ANOVA followed with Sidak’s multiple comparisons test (c) or Student’s two-tailed unpaired t-test (e and g).
