Extended Data Fig. 7: PKM2 expression is higher in the neuronal somata than that in the terminals.
a-h, Distribution of PKM1 (yellow) and PKM2 (red) in the somata and nerve terminals in the substantia nigra (a), locus ceruleus (c), diagonal band nucleus (e) and cerebellum (g) of 2-month-old C57BL/6 J mice. The somata were labelled with the tyrosine hydroxylase (TH) (a and c), choline acetyltransferase (CHAT) (e) and calbindin (CAL) (g) antibody (green). The terminals were labelled with the synaptophysin (SYP) antibody (blue). Nuclei were labelled with DAPI (cyan). Quantification of PKM1 and PKM2 (p < 0.0001) fluorescence intensity in the somata (n = 259) and nerve terminals (n = 339) in the substantia nigra (b). Quantification of PKM1 (p < 0.0001) and PKM2 (p < 0.0001) fluorescence intensity in the somata (n = 176) and nerve terminals (n = 213) in the locus ceruleus (d). Quantification of PKM1 and PKM2 (p < 0.0001) fluorescence intensity in the somata (n = 119) and nerve terminals (n = 162) in the diagonal band nucleus (f). Quantification of PKM1 and PKM2 (p < 0.0001) fluorescence intensity in the somata (148) and nerve terminals (207) in the cerebellum (h). For the boxplots, the centre line indicates the median, the box limits indicate the first and third quartiles, and the whiskers indicate the data range. Scale bars, 20 μm. ***p < 0.001. Two-way ANOVA followed with Sidak’s multiple comparisons test (b, d, f and h).
