Extended Data Fig. 1: Identification of isolated neuronal somata and synaptosomes. | Nature Neuroscience

Extended Data Fig. 1: Identification of isolated neuronal somata and synaptosomes.

From: Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage

Extended Data Fig. 1

a, Typical electron microscopy image of synaptosomes, containing mitochondria (m) and synaptic vesicles (sv). b, Quantitative analysis of the supernatant glutamate (Glu, p < 0.0001) and γ-aminobutyric acid (GABA, p = 0.0144) after the synaptosomes were treated with 30 mM KCl for 5 min (n = 6 biological replicates). c, AO/PI staining of isolated neuronal somata. Left, bright field of isolated neuronal somata. Right, green arrows indicate living somata; orange arrows indicate apoptotic somata; red arrows indicate necrotic somata. d, Statistical analysis of apoptotic and necrotic neuronal somata (n = 6 biological replicates). e, Capillary western blot analysis of synaptic markers (VAMP and synaptophysin (SYP)), nuclear component (Histone), and glial cell markers (GFAP and Iba1) in the whole brain homogenates (H), somata (S) or nerve terminals (T) (n = 3 independent experiments). Samples were normalised to the total protein indicated by blue circles. Scale bars, 200 nm (a) and 200 μm (c). Data are presented as mean ± SEM and *p < 0.05 and ***p < 0.001. Two-way ANOVA followed with Sidak’s multiple comparisons test (b).

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