Extended Data Fig. 3: Different glucose metabolism between the somata and nerve terminals in firing neurons.
a, Left, representative images show a patched cell at 4× magnification (top) and 40× magnification (bottom) differential interference contrast microscopy in a mouse SSp brain slice. Right, whole-cell recordings showing continuous firing of the pyramidal neuron in the SSp following 100 pA current injection (n = 15 independent experiments). b, The somatic iATPSnFR fluorescence of neurons in the SSp brain slice was measured before and after a 5 s injection of 100 pA current. The brain slice was treated separately with aCSF, aCSF containing 10 μM Oligomycin A (p = 0.0042), or glucose-free aCSF containing 10 μM Oligomycin A and 3.5 mM 2-DG (p < 0.0001), n = 15 from 3 mice. c, The nerve terminal calcium signals in the SSp brain slice evoked by a 30 s burst of 10 Hz field stimulation. Synaptophysin-mCherry was expressed to locate nerve terminals. The horizontal line represents the duration of the 30 s field stimulation (n = 28 from 3 mice). d, The terminal iATPSnFR fluorescence of neurons in the SSp brain slice was measured before and after a 30 s burst of 10 Hz field stimulation. The brain slice was treated separately with aCSF, aCSF containing 10 μM Oligomycin A (p < 0.0001), or glucose-free aCSF containing 10 μM Oligomycin A and 3.5 mM 2-DG (p < 0.0001), n = 55 from 3 mice. e, The OXPHOS index (OXPHOS/(OXPHOS + glycolysis)) in somata (n = 15 from 3 mice) and nerve terminals (n = 55 from 3 mice), p < 0.0001. For the boxplots, the centre line indicates the median, the box limits indicate the first and third quartiles, and the whiskers indicate the data range. Data are presented as mean ± SEM and **p < 0.01 and ***p < 0.001. Student’s two-tailed unpaired t-test (b, d and e).
