Extended Data Fig. 4: Spatial expression of markers for the different MN clusters.
From: Molecular blueprints for spinal circuit modules controlling locomotor speed in zebrafish

a, RNAscope in situ hybridization of grin1b and neurod1 in slow–intermediate and fast MNs. b, Normalized soma position of Grin1b+ and Pvalb6+ MNs in the spinal cord. The size of the circles reflects the number of puncta per cell (# puncta) (n = 5 animals). c, RNAscope in situ hybridization of pvalb6 and chrna2b in slow–intermediate or fast MNs. d, Normalized soma position of Pvalb6+ and Chrna2b+ MNs in the spinal cord. The size of the circles reflects the number of puncta per cell (# puncta) (n = 3 animals). e, RNAscope in situ hybridization of pvalb6 and neurod1 in slow–intermediate or fast MNs. f, Normalized soma position of Pvalb6+ and Neurod1+ MNs in the spinal cord. The size of the circles reflects the number of puncta per cell (# puncta) (n = 3 animals). g, RNAscope in situ hybridization of neurod1 and calb1 in slow–intermediate or fast MNs. h, Normalized soma position of Neurod1+ and Calb1+ MNs in the spinal cord (n = 4 animals). i, Number of puncta of each RNAscope probe in slow–intermediate and fast MNs (Two-tailed Mann–Whitney U test, ****p < 0.0001; Grin1b: n = 9 animals; Pvalb6: n = 10 animals; Chrna2b: n = 7 animals; Neurod1: n = 12 animals; Calb1: n = 9 animals).