Extended Data Fig. 4: Spatial expression of markers for the different MN clusters. | Nature Neuroscience

Extended Data Fig. 4: Spatial expression of markers for the different MN clusters.

From: Molecular blueprints for spinal circuit modules controlling locomotor speed in zebrafish

Extended Data Fig. 4

a, RNAscope in situ hybridization of grin1b and neurod1 in slow–intermediate and fast MNs. b, Normalized soma position of Grin1b+ and Pvalb6+ MNs in the spinal cord. The size of the circles reflects the number of puncta per cell (# puncta) (n = 5 animals). c, RNAscope in situ hybridization of pvalb6 and chrna2b in slow–intermediate or fast MNs. d, Normalized soma position of Pvalb6+ and Chrna2b+ MNs in the spinal cord. The size of the circles reflects the number of puncta per cell (# puncta) (n = 3 animals). e, RNAscope in situ hybridization of pvalb6 and neurod1 in slow–intermediate or fast MNs. f, Normalized soma position of Pvalb6+ and Neurod1+ MNs in the spinal cord. The size of the circles reflects the number of puncta per cell (# puncta) (n = 3 animals). g, RNAscope in situ hybridization of neurod1 and calb1 in slow–intermediate or fast MNs. h, Normalized soma position of Neurod1+ and Calb1+ MNs in the spinal cord (n = 4 animals). i, Number of puncta of each RNAscope probe in slow–intermediate and fast MNs (Two-tailed Mann–Whitney U test, ****p < 0.0001; Grin1b: n = 9 animals; Pvalb6: n = 10 animals; Chrna2b: n = 7 animals; Neurod1: n = 12 animals; Calb1: n = 9 animals).

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