Extended Data Fig. 6: Analysis of TFs and regulons in the V2a IN dataset.
From: Molecular blueprints for spinal circuit modules controlling locomotor speed in zebrafish
a, UMAP of four color-coded TF-defined V2a IN clusters (V2a-t1-4). The cluster in gray corresponds to the immature V2a IN cluster (V2a4). The inset shows the whole dataset-defined clusters from Fig. 1. b, Examples of differentially expressed TFs in each TF-defined V2a IN cluster. The size of the circle reflects the proportion of the cells expressing the gene in a cluster (% expressed), and the color intensity reflects its average expression level within that cluster (avg. expressed). c, Plots of highly enriched regulons in each TF-defined V2a IN cluster. d, Table showing regulons with highest normal enrichment scores (NES) obtained by iRegulon analysis. A high NES (>3.0) indicates a TF that most likely acts as a regulator of a subset of target genes sharing specific RNA binding motifs. e, Heatmap (left) and Sankey plot (right) of differentially expressed genes shared by different TF-defined V2a IN and MN clusters.
