Extended Data Fig. 7: NLGN2 and gephyrin immunoreactivity are lacking at P4. | Nature Neuroscience

Extended Data Fig. 7: NLGN2 and gephyrin immunoreactivity are lacking at P4.

From: The developmental timing of spinal touch processing alterations predicts behavioral changes in genetic mouse models of autism spectrum disorders

Extended Data Fig. 7

Related to Fig. 6. a, b, Spinal cord immunohistochemistry of VGAT and NLGN2 in P4 and adult animals. Scale bars denote 5 µm. c, Quantification of VGAT/NLGN2 co-labeling. d, e, Spinal cord immunohistochemistry of VGAT and gephyrin in P4 and adult animals. Scale bars denote 5 µm. f, Quantification of VGAT/gephyrin co-labeling. For c and f, Each dot represents the average value for one animal, with three spinal cord images taken per animal. N = 3 P3-P4 mice and N = 7 adult mice for NLGN2, and N = 3 P3-P4 mice and N = 4 adult mice for gephyrin. Data represent means ± s.e.m. g, Proposed model for the molecular and circuit development of presynaptic inhibition (PSI) and feedforward inhibition (FFI) in the spinal cord. Glu denotes glutamate, and GlyR denotes glycine receptor.

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