Fig. 2: Neonatal tactile reactivity in global knockout ASD mouse models.
a, Diagram showing the experimental paradigm for the neonatal air puff responsivity assay. Air puff tubing was affixed 3 mm above the nape of the neck of neonatal mice. Maximal body displacements in the 500 ms following a 50-ms air puff were measured using optic flow analysis. Parts were created with BioRender.com. b, Example displacement trace of a control P4 mouse to a single 1.0-psi air puff stimulus. c, Displacement responses to a single presentation of 0.10-, 0.25-, 0.50-, 0.75- and 1.0-psi stimuli; n = 16 Gabrb3+/+ mice and n = 10 Gabrb3+/– mice; n = 12 Mecp2+/y mice and n = 11 Mecp2–/y mice; n = 17 Nlgn2+/+ mice and n = 15 Nlgn2+/– mice; n = 18 Rorb+/+ mice and n = 17 Rorbh1/+ mice. Data were analyzed by mixed-effects analyses, effect of genotype; Gabrb3: F1,24 = 9.060 and P = 0.0030; Mecp2: F1,21 = 9.253 and P = 0.0062; Nlgn2: F1,30 = 0.1071 and P = 0.7457; Rorb: F1,33 = 0.1204 and P = 0.7308. Data are shown as mean ± s.e.m. d, Average displacement responses to ten presentations of a 1.0-psi stimulus at 20- to 30-s interstimulus intervals; n = 16 Gabrb3+/+ mice and n = 10 Gabrb3+/– mice; n = 12 Mecp2+/y mice and n = 11 Mecp2–/y mice; n = 17 Nlgn2+/+ mice and n = 15 Nlgn2+/– mice; n = 18 Rorb+/+ mice and n = 17 Rorbh1/+ mice. P = 0.0003 for Gabrb3, P = 0.0018 for Mecp2, P = 0.2258 for Nlgn2 and P = 0.1970 for Rorb. Data were analyzed by unpaired one-tailed t-tests and are shown as mean ± s.e.m. e, Example traces showing displacements to ten presentations of 1.0-psi stimuli from a Gabrb3+/+ and Gabrb3+/– mouse. To calculate habituation, the average of the last three stimulus presentation responses was compared to the average of the first three responses. A >25% reduction in responsivity between blocks was considered to be a habituation response. f, Fraction of animals habituating to repeated presentations of 1.0-psi air puffs; P = 0.0244 for Gabrb3, P = 0.0317 for Mecp2; P = 0.7258 for Nlgn2 and P = 0.6017 for Rorb. Data were analyzed by one-sided Fisher’s exact tests. Statistical information is provided in Supplementary Table 1; *P < 0.05; **P < 0.001.
