Extended Data Fig. 4: Projection and cell type-specific ChR2 interrogation of the PnO → Gi pathway.
From: Basal ganglia–spinal cord pathway that commands locomotor gait asymmetries in mice
a, Strategy for stimulating Vglut2+ neurons in PnO which receive input from SNripsi and project to Gicontra. In Vglut2Cre mice, AAVretro-con/fon-ChR2 was injected in Gileft followed by AAV1-FlpO in SNrright. An optic fiber (O.f.) was then implanted over PnO. b, Optogenetic stimulation of PnO-Vglut2contra neurons using the strategy in (a) evoked contraversive turning. Body, ***P = 5.7 × 10−4; head, P = 0.90; one-way ANOVA with Tukey’s multiple comparison test; n = 6 mice (where n is the average of 10 trials for each mouse) from one experiment. c, Strategy for stimulating Chx10+ neurons in Gi which receive input from the PnOcontra. In Chx10Cre mice, AAV1-FlpO was injected in PnOright followed by AAV-con/fon-ChR2 in Gileft. An optic fiber was then implanted over Gi. d, Optogenetic stimulation of PnO recipient, Chx10+ Gi neurons evoked ipsiversive turning. Body, ***P = 1.3 × 10−4; head, P = 0.80; one-way ANOVA with Tukey’s multiple comparison test; n = 6 mice (where n is the average of 10 trials for each mouse) from one experiment. Error bands in the time series plots in b and d (top) represent the standard error of the mean. Box-and-whisker plots in b and d give the median, the 25th and 75th percentiles, and the range.
