Fig. 6: Restoration of contraversive turning gaits in mice with acute unilateral striatal dopamine depletion.
From: Basal ganglia–spinal cord pathway that commands locomotor gait asymmetries in mice
a, Strategy for restoring contraversive turning in mice with unilateral striatal dopamine depletion. b, Unilateral 6-OHDA-lesioned mice exhibited loss of dopaminergic (TH-positive, TH+) terminals in the ipsilateral striatum, and loss of TH+ neurons of the ipsilateral SNc. c, Quantification of 6-OHDA lesion efficacy. n = 10 mice from 2 independent experiments. d, Analysis of left versus right movement preference in the open field. −CNO/−6-OHDA versus +CNO/−6-OHDA, ***P = 8.3 × 10−11; −CNO/−6-OHDA versus −CNO/+6-OHDA, ***P = 1.7 × 10−4; −CNO/+6-OHDA versus +CNO/+6-OHDA, ***P < 1.0 × 10−15; two-way ANOVA with Tukey’s multiple comparison test; n = 10 mice from 2 independent experiments. Pre-lesion data (−6-OHDA) are also presented in Extended Data Fig. 6d. e, Mice with acute unilateral striatal dopamine depletion exhibited predominantly ipsiversive (right) movement preference. CNO administration at t = 0 reversed ipsiversive rotational biases in a time-dependent manner. Error bands represent the s.e.m. f, CNO left versus CNO right, ***P = 9.0 × 10−4; two-way ANOVA with Tukey’s multiple comparison test; n = 10 mice from 2 independent experiments. g, Strategy for testing the efficacy of PnO-Vglut2contra neurons in restoring contraversive turning in mice with acute unilateral striatal dopamine depletion. h, Optogenetic stimulation of PnO-Vglut2contra neurons restored straight locomotion at 5 Hz and 10 Hz and contralateral turning at 20 Hz and 40 Hz. i, Mock versus 5 Hz, *P = 0.02; mock versus 10 Hz, *P = 0.018; mock versus 20 Hz, *P = 0.012; mock versus 40 Hz, *P = 0.018; one-way repeated measures ANOVA with Tukey’s multiple comparison test; n = 4 mice (where n is the average of 10 trials for each mouse) from 1 experiment. j, Example trajectories associated with mock or 1-s ChR2 stimulation. k, Trial-by-trial analysis of angular velocity. n = 4 mice (10 trials per mouse) from 1 experiment. Box-and-whisker plots in c, d, f and i give the median, the 25th and 75th percentiles and the range.
