Extended Data Fig. 2: Stereotypic modulation of Chx10 Gi activity via unilateral activation of D2 SPNs.
From: Basal ganglia–spinal cord pathway that commands locomotor gait asymmetries in mice
a, Schematic of Chx10 Gileft GCaMP7s recording with optogenetic stimulation of left or right D2 SPNs. b, Stimulation of D2 SPNs caused ipsiversive turns, with an increase in Chx10 Gi activity ipsilateral to the stimulation and a decrease in Chx10 Gi activity contralateral to the stimulation. n = 7 mice from two independent experiments; 10 left and right D2 stimulation trials for each mouse. Error bands represent the standard error of the mean. c, Top, Change in direction associated with 1 s ChR2 stimulation of left or right D2 SPNs. Bottom, Change in Chx10 Gileft ΔF/F (dF/F) activity associated with 1-s ChR2 stimulation of left or right D2 SPNs. The magnitude of the change in ΔF/F was similar for D2right versus D2left stimulation trials. P = 0.81; two-tailed paired t-test; n = 7 mice from two independent experiments. Violin plots give the median, the 25th and 75th percentiles, and the range. See Supplementary Table 1 for full statistical analysis. d, Model for locomotor asymmetries caused by stimulation of D2 SPNs. Optogenetic stimulation of D2 SPNs has a contralateral inhibitory effect on Chx10 Gi neurons, as well as an ipsilateral excitatory effect.
