Extended Data Fig. 2: TREM1 amplifies levels of inflammatory factors but does not alter phagocytosis.
a Quantification of immune factors in lysates of peritoneal MΦ isolated from 8.5 mo WT and Trem1-/- mice treated with vehicle or 100 ng/ml LPS for 20 hr. Two-way ANOVA with Tukey’s multiple comparisons shown for WT + LPS vs Trem1-/-+ LPS (n = 5–6 male mice per genotype as shown). b Trem1 deficiency does not alter E.coli phagocytosis in peritoneal MΦ from 2–3 mo male mice. Cells were stimulated with vehicle or increasing concentrations of LPS for 20 h. 2-way ANOVA, effect of LPS ***P < 0.001, no effect of Trem1 genotype basally or with LPS stimulation on 6–11 technical replicate wells as shown.
