Extended Data Fig. 3: TREM1 promotes a pro-inflammatory polarization state in aged peritoneal MΦ.

a Gating strategy for live Cd11b⁺Ly6G⁻CD45⁺Ly6C^Hi and Cd11b⁺Ly6G⁻CD45⁺Ly6C^Lo peritoneal MΦ from young WT and Trem1^-/- (2 mo) and aged WT and Trem1^-/- (22–23 mo) male mice. b Representative gating for MHC II, CD86, and CD71 positive Ly6C^Hi peritoneal MΦ. c Representative gating for MHC II, CD86, and CD71 positive Ly6C^Lo peritoneal MΦ. d Multianalyte Luminex quantification of immune factors in young WT (2 mo) and aged WT (23–24 mo) and aged Trem1^-/- (23–24 mo) cerebral cortex. One-way ANOVA with Tukey’s multiple comparisons shown (n = 5–6 male mice per group as shown). e Total time exploring (in seconds) during the training phase of the NOR task; ANOVA with Tukey’s multiple comparisons show no significant differences (n = 6–8 female mice as shown). f Speed (pixels/second) of mice during the final trial of the Barnes Maze; ANOVA with Tukey’s multiple comparisons (n = 5–6 male and female mice as shown).

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