Extended Data Fig. 4: PL-MS identifies the dendritic proteome and its relation to local RNA levels.
From: Neuronal activity rapidly reprograms dendritic translation via eIF4G2:uORF binding
a, PCA plot showing five replicates of PL-MS from Pan-TurboID and TurboID-PSD95-transduced resting and depolarized neurons. Minus biotin counterpart of each sample is subtracted for each dot on the plot. b, Comparison of PL-MS-enriched proteome with previously published dataset13 (FDR < 0.05). c, TurboID-PSD95-enriched proteome vs. Distler et al. data51 (FDR < 0.05). Multiple testing correction was performed using the Benjamini-Hochberg method. d, Pearson correlation of PL-MS and PL-CLIP on dendritic RNAs in resting neurons. e, Shown are the distributions of PL-MS-enriched dendritic proteome from resting (rest), depolarized (dep) and differential (dep minus rest) data in ranked resting PL-CLIP using a GSEA-based strategy (FDR < 0.05) (Methods). Multiple testing correction was performed using the Benjamini-Hochberg method in the fgsea package. P adjusted: Rest = 0.0026, Dep = 0.18, Dep-Rest = 0.0027. P values: ** <0.01. f, Pearson correlation of PL-MS and PL-CLIP on dendritic RNAs in depolarized neurons. (d,f) The reported P values are two-sided.
