Extended Data Fig. 6: Macromolecular content in cytomegalic and normal-appearing FCDII cells. | Nature Neuroscience

Extended Data Fig. 6: Macromolecular content in cytomegalic and normal-appearing FCDII cells.

From: Targeting pathological cells with senolytic drugs reduces seizures in neurodevelopmental mTOR-related epilepsy

Extended Data Fig. 6

(a) (Left) Toluidine blue staining showing a cytomegalic cell with a soma diameter >25μm (white arrow) and a normal-appearing cell (red arrow) in an MTOR-related FCDIIb tissue (ID#3). (Right) Electron microscopy images of the intracellular content of a cytomegalic cell from the same tissue. Orange shade: lysosomes. Purple shade: multivesicular bodies. (b) Representative images of cytomegalic cells in n = 5 FCDII resected brain tissues showing abnormal accumulation of electron-dense enlarged lysosomes (orange arrows) and multivesicular bodies (purple arrows). Patient IDs (left to right, top to bottom): #12, 3, 4, 7, 11, 3. (c) Representative images of normal-appearing cells in the same panel of n = 6 FCDII cases showing typical cytoplasmic content. Patient IDs (left to right, top to bottom): #11, 7, 3, 11, 12, 3. Electron microscopy imaging was repeated at least three times.

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