Fig. 4: Clearance of senescent cells after DQ administration in Mtor^S2215F animals.

a, Experimental design of IUE targeting layer 2/3 pyramidal cell-destined progenitor cells lining the dorsal ventricular zone at E14.5 and subsequent localization of the electroporated area in one hemisphere. b, Study design for IUE, histological and biochemical experiments. c, Top, representative images of immunofluorescent staining against pS6 (red) and DAPI (blue) on Mtor^S2215F mice after vehicle or DQ administration. Bottom, quantification of pS6⁺ cell density and pS6⁺ cell mean fluorescence intensity on n = 6 vehicle-treated and n = 6 DQ-treated Mtor^S2215F animals (each dot corresponds to one animal). **P = 0.0022 and NS, not significant, two-tailed Mann–Whitney test. d, Top, representative bright-field images of SAβGal colorimetric assay (blue) on Mtor^S2215F animals after vehicle or DQ administration in ipsilateral and controlateral regions. Bottom, quantification of SAβGal⁺ cell density in ipsilateral on n = 4 vehicle-treated and n = 4 DQ-treated Mtor^S2215F animals (each dot corresponds to one animal). e, Western blot on electroporated cortical brain lysates against pS6, p53 and p19 from n = 3 vehicle-treated and n = 3 DQ-treated Mtor^S2215F mice. Histogram showing the relative expression of pS6, p53 and p19 to actin (each dot corresponds to one animal). f, Histograms showing the quantification of canonical SASP cytokine production in the same brain lysates as d of n = 3 vehicle-treated and n = 3 DQ-treated Mtor^S2215F animals (averaging two technical replicates per animal). Values are normalized to the mean of vehicle-treated Mtor^S2215F animals. Scatter dot plots are presented as mean ± s.e.m. fluo., fluorescence; nb, number of; Veh, vehicle; w, weeks.

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