Extended Data Fig. 1: Cortical activity and dysmorphic neuron topography in human FCDIIb brain cortical slices.
(a) Representative delineation of gray-white matter boundary. GM: gray matter, WM: white matter. (b-e) (Left) Micrographs of cortical slices of resected tissue from FCDIIb patients showing multielectrode array (MEA) layout. Electrodes are color-coded to show the type of activity detected (Red: IILD + MUA; Orange: MUA only; Green: no activity; Gray: damaged electrode). Scale bar: 2 mm. (Right) Immunofluorescent staining from the corresponding cortical slice with antibodies against pS6 (red) and NeuN (green) in regions delineated by color-coded dashed lines on MEA layout. Dysmorphic neurons (DNs) are identified by horizontal white arrows and balloon cells (BCs) are identified by vertical yellow arrows. d: density = number of DNs/mm2. Cortical slices represented are: (b) Slice #2 from patient ID#8 (for which no immunofluorescent staining is available); (c) Slice #1 from patient ID#8; (d) Slice #4 from patient ID#8; (e) Slice #1 from patient ID#9. MEA recordings and histology stainings on the same sections could only be performed once on each slice.
