Extended Data Fig. 2: Enhanced prion neurotoxicity in transiently NG2-glia-depleted Tga20 COCS.
a, Experimental design of transient NG2 glia depletion in Tga20 COCS. b, NeuN and NG2 immunofluorescence showing enhanced neurodegeneration in transiently NG2-glia-depleted (CP673451) Tga20 COCS compared to NG2 glia intact (DMSO) Tga20 COCS after prion infection. NG2 glia density is largely recovered in prion-infected, transiently NG2-glia-depleted COCS at the end of the experiment. Nuclei were stained with DAPI (blue). c, Quantification of NG2 immunointensity and NeuN positive area shown in b. NeuN: n = 20 slices for NBH + DMSO and NBH + CP673451; n = 28 slices for Prion+DMSO; n = 31 slices for Prion+CP673451. NG2: n = 12 slices for NBH + DMSO; n = 16 slices for Prion+DMSO, NBH + CP673451 and Prion+CP673451. Data are presented as mean ± SEM. One-way ANOVA with Benjamini-Hochberg FDR adjustment for multiple comparisons. NeuN: P = 0.1057 (NBH + DMSO vs. NBH + CP673451); P < 0.0001 (NBH + DMSO vs. Prion+DMSO); P < 0.0001 (Prion+DMSO vs. Prion+CP673451). NG2: P = 0.0690 (NBH + DMSO vs. NBH + CP673451); P < 0.0001 (NBH + DMSO vs. Prion+DMSO); P = 0.6641 (Prion+DMSO vs. Prion+CP673451).
