Extended Data Fig. 7: Cell-cell communications from NG2 glia to microglia in the hippocampus of prion-inoculated mice.

a, UMAP of single-cell RNA-seq data showing Cox2⁺ and Ptges⁺ microglia among total microglia in the hippocampus of prion- or NBH-inoculated mice. b, Quantification of Cox2⁺ and Ptges⁺ microglia fractions against total microglia in the hippocampus of prion- or NBH-inoculated mice shown in a. n = 2 mice for NBH and n = 7 mice for Prion. Data are presented as mean ± SEM. Unpaired t test (two-sided): P = 0.0216 (Cox2⁺ microglia); P = 0.0116 (Ptges⁺ microglia). c, Venn diagram showing numbers of shared and distinct DEGs of Cox2⁺ and Ptges⁺ microglia in the hippocampus of prion-inoculated mice. d, Heatmap showing downregulation of homeostatic microglia signature genes and upregulation of DAM and MHC-II microglia signature genes as well as inflammatory genes in Cox2⁺ and Ptges⁺ microglia in the hippocampus of prion-inoculated mice. e, CellChat analysis of cell-cell communications showing unaltered number of interactions (plotted as the thickness of the edges) but reduced strength of interactions (plotted as the thickness of the edges) from NG2 glia to Cox2⁺ microglia in the hippocampus of prion-inoculated mice. f, Heatmaps showing significantly weakened NG2 glia to Cox2⁺ microglia interaction pathways in the hippocampus of prion-inoculated mice.

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