Fig. 3: Loss of NG2 glia upregulates Cox2–Ptges expression.
a,b, NG2 immunofluorescence (a) and quantification (b) in intact (DMSO) and NG2-glia-depleted (CP673451) C57BL/6J COCS; n = 7 slices per condition. Data are presented as mean ± s.e.m. Unpaired t-test (two-sided): P < 0.0001. c, qRT-PCR results showing downregulation of NG2 and Pdgfrα and upregulation of Cox2 and Ptges in NG2-glia-depleted (CP673451) C57BL/6J COCS; n = 6 samples. Data are presented as mean ± s.e.m. Multiple unpaired t-tests with Benjamini–Hochberg FDR adjustment for multiple comparisons. NG2: P < 0.0001; Pdgfrα: P < 0.0001; Cox2: P < 0.0001; Ptges: P = 0.0067. d,e, NG2 immunofluorescence (d) and quantification (e) in control (Ctrl) and PDGFAA-treated C57BL/6J COCS; n = 7 slices per condition. Data are presented as mean ± s.e.m. Unpaired t-test (two-sided): P < 0.0001. f, qRT-PCR results showing upregulation of NG2 and Pdgfrα and downregulation of Cox2 and Ptges in C57BL/6J COCS with increased NG2 glia density (PDGFAA) compared with NG2-glia-intact (Ctrl) C57BL/6J COCS; n = 4 samples for Ctrl; n = 6 samples for PDGFAA. Data are presented as mean ± s.e.m. Multiple unpaired t-tests with Benjamini–Hochberg FDR adjustment for multiple comparisons. NG2: P = 0.0018; Pdgfrα: P < 0.0001; Cox2: P = 0.0003; Ptges: P < 0.0001. g, qRT-PCR results showing upregulation of Cox2 and Ptges in NG2-glia-depleted (CP673451) Tga20 COCS after prion exposure; n = 6 samples. Data are presented as mean ± s.e.m. Unpaired t-test (two-sided): P = 0.0078 for Cox2; P = 0.0060 for Ptges. h, Cox2, Ptges and Iba1 immunofluorescence in the hippocampus showing upregulation of Cox2 and Ptges and their colocalization with microglia in brains of terminally sick prion-inoculated mice. i, Quantification of Cox2 and Ptges immunointensity shown in h; n = 3 mice for NBH and n = 4 mice for prion. Data are presented as mean ± s.e.m. Unpaired t-test (two-sided): P = 0.0099 for Cox2; P = 0.0006 for Ptges. j, Cox2 and Ptges immunofluorescence in the hippocampus showing upregulation of Cox2 and Ptges in the brains of NG2-glia-depleted (PdgfrαiDTR) mice compared with NG2-glia-intact (Ctrl) mice after prion inoculation. NG2 glia depletion was induced at 16 wpi; brain samples were collected at 21 wpi. k, Quantification of Cox2 and Ptges immunointensity shown in j; n = 6 mice per group. Data are presented as mean ± s.e.m. Unpaired t-test (two-sided): P = 0.0048 for Cox2; P = 0.0015 for Ptges.
