Extended Data Fig. 1: Enhanced prion neurotoxicity in continuously NG2-glia-depleted C57BL/6 J COCS.
a, NG2 immunofluorescence showing NG2 glia activation in prion-infected C57BL/6 J COCS vs C57BL/6 J COCS exposed to NBH. Nuclei were stained with DAPI (blue). b, Quantification of NG2 immunointensity shown in a. n = 8 slices for NBH; n = 11 slices for Prion. Data are presented as mean ± SEM. Unpaired t test (two-sided): P = 0.0007. c-d, Experimental design of continuous NG2 glia depletion in Tga20 COCS (c) and C57BL/6 J COCS (d). e, NeuN and NG2 immunofluorescence showing enhanced neurodegeneration in continuously NG2-glia-depleted (CP673451) C57BL/6 J COCS compared to NG2 glia intact (DMSO) C57BL/6 J COCS after prion infection. Nuclei were stained with DAPI (blue). f, Quantification of NG2 immunointensity and NeuN positive area shown in e. n = 14 slices/condition for NeuN; NG2: 8 slices for NBH + DMSO; 10 slices for Prion+DMSO; 15 slices for NBH + CP673451 and Prion+CP673451. Data are presented as mean ± SEM. One-way ANOVA with Benjamini-Hochberg FDR adjustment for multiple comparisons. NeuN: P = 0.2466 (NBH + DMSO vs. NBH + CP673451); P = 0.0035 (NBH + DMSO vs. Prion+DMSO); P = 0.0288 (Prion+DMSO vs. Prion+CP673451). NG2: P < 0.0001 (NBH + DMSO vs. NBH + CP673451); P < 0.0001 (NBH + DMSO vs. Prion+DMSO); P < 0.0001 (Prion+DMSO vs. Prion+CP673451).
