Extended Data Fig. 9: Single nucleus RNA sequencing of control and Arl13b^cKO cortices.

(a) UMAP plot of all cells from single nucleus RNA sequencing. (b) Density plot of representative marker genes used for cell type annotation. (c) Density plot of markers used to identify upper layers (Layer II/III, Layer IV) and deeper layers (Layer V, Layer VI) excitatory neurons and inhibitory neurons. (d) CellChat predicted number of interactions from astrocytes to other cell types in control and Arl13b^cKO groups. (e) CellChat generated dot plot showing the significant ligand-receptor pairs between Control and Arl13b^cKO brains, which contribute to the signaling from astrocytes to other cell types. Dot color reflects communication probabilities and dot size represents P values computed using a one-sided permutation test. (f) CellChat predicted altered signaling interaction patterns in Arl13b^cKO brains.