Extended Data Fig. 3: Single cell RNAseq analyses of Ift88^cKO cortical astrocytes.

(a) Kernel density estimates depicting magnitude of the molecular changes in Ift88^cKO calculated by summing DEG FCs for each cortical astrocyte cluster. (b) Venn diagrams of DEGs found in Arl13b^cKO and Ift88^cKO groups (compared to Control) across cortical astrocyte Cluster 0, 1, 2. (c) Visualization of top DEGs in Ift88^cKO cortical astrocyte clusters. DEGs found using the function of FindMarkers in Seurat R package and were defined significant when P_adj < 0.05. (d-f) Top GO pathways significantly altered (P < 0.05) in Arl13b^cKO and Ift88^cKO cortical astrocyte clusters. The insignificant GO terms are indicated as P > 0.05. (g) The influence of Ift88 deletion on the key GO pathways in cortical astrocyte clusters. GSEA was used for statistical analysis in (d-g).