Fig. 3: EGL-43 is a conserved ZF TF that controls presynaptic gene expression.
From: An activity-regulated transcriptional program directly drives synaptogenesis
a, Aggregation plot of EGL-43 ChIP–seq signal across C. elegans genes. b, Aggregation plot of EGL-43 dopaminergic-specific NanoDam signal across C. elegans genes. For a and b, bold lines represent the mean signal, darker shading represents the estimated s.e. and lighter shading represents the 95% confidence interval. c, Venn diagram comparing EGL-43 ChIP–seq and dopaminergic-specific NanoDam datasets. Numbers represent peaks, either overlapping or unique to each dataset. d, GO analysis performed on the overlapping peaks between ChIP–seq and NanoDam datasets (PANTHER biological process). e, JASPAR TF binding profile for mouse (M. musculus) MECOM. f, Proportion of peaks within EGL-43 profiling datasets that contain a 5′-AAGATAA-3′ motif. g, Line scans of three PDE axons in animals carrying FLP-on ELKS-1 in the wild type and elks-1(wy1823), which possess a mutant EGL-43 motif. h, Quantification of PDE axon line scans of animals shown in f. P = 2.50832 × 10−6 and was calculated using a two-tailed unpaired Student’s t test. n = 15 for both genotypes. Medians are represented in thick dashed lines, and quartiles are represented in thin dashed lines. i, Representative micrographs of endogenous DAT-1 signal in animals carrying EGL-43::TagRFP-T::AID*. Animals were subjected to auxin treatment for 48 h. Arrowheads denote cell bodies of dopaminergic neurons (white, 0 mM auxin, and pink, 4 mM auxin). Numbers indicate the number of animals that resemble the phenotype shown in images. This experiment was repeated four times with similar penetrance. j, Representative micrographs of endogenous DAT-1 signal in wild-type animals and animals and dat-1(wy1824), which possess a mutant EGL-43 motif. White and yellow arrowheads indicate dopaminergic neuron cell bodies of respective genotypes. Numbers indicate the number of animals that resemble the phenotype shown in images. This experiment was repeated thrice with similar penetrance. Scale bar = 10 μm for all images.
