Fig. 4: FOS-1 is critical for presynaptic gene expression in dopaminergic neurons.
From: An activity-regulated transcriptional program directly drives synaptogenesis
a, Images of L4 animals carrying endogenous FOS-1::GFP and EGL-43::TagRFP-T::AID* treated with 0 mM or 4 mM auxin for 48 h. PVD and PDE neuronal nuclei are outlined (white dashed circles). Auxin experiments were repeated independently with similar results (n = 3). b, Quantification of FOS-1::GFP intensity of nuclei (n = 15 for both conditions). c, Venn diagram displaying overlap of peaks between dopaminergic FOS-1 NanoDam and EGL-43 NanoDam. d, Left: GO term analysis of genes mapping to FOS-1 NanoDam peaks. Right: GO term analysis of genes mapping to the overlap between FOS-1 and EGL-43 NanoDam peaks. e, Line scans of FLP-on GFP ELKS-1 in PDE axons. The top two sets of line scans correspond to animals carrying endogenous FOS-1::TagRFP-T::AID* that were treated with either 0 mM or 4 mM auxin. Images were thresholded in the same fashion. The line scan denoted with single asterisk was thresholded to increase the visibility of synapses for illustrative purposes. Arrowheads point to small synapses that are nearly undetectable in the distal axon. The final set of PDE line scans corresponds to FLP-on ELKS-1 expression in animals with the AP-1 site deleted upstream of the TSS of elks-1. f, Quantification of line scans in e, where n = 14 for all conditions. For graph comparisons, P = 5.77664 × 10−21 for auxin (−) versus auxin (+) and P = 8.76057 × 10−21 for auxin (−) versus ΔAP-1 site. g, Line scans of FLP-on GFP RAB-3 in PDE axons of animals carrying FOS-1::TagRFP-T::AID*, treated with 0 mM or 4 mM auxin. h, Quantification of animals i (n = 15 for both conditions, P = 5.21856 × 10−8). For all graphs, medians are represented in thick dashed lines, and quartiles are represented in thin dashed lines. P values were calculated using two-tailed unpaired Student’s t tests. Scale bar = 10 μm for all images.
