Fig. 5: FOS-1 modulates egl-43 expression.
From: An activity-regulated transcriptional program directly drives synaptogenesis
a, L4 animals carrying EGL-43::GFP and FOS-1::TagRFP-T::AID* were treated with 0 mM or 4 mM auxin for 48 h to degrade FOS-1. PVD and PDE nuclei are outlined (white dashed circles). b, Quantification of EGL-43::GFP intensity shown in a (n = 16 for both conditions). c, JASPAR TF binding profiles for C. elegans FOS-1 (top) and H. sapiens FOS (bottom). d, SNP effect matrix of H. sapiens FOS. The purple rectangle highlights the position of the consensus FOS motif that differs in wild-type C. elegans. e, EGL-43::GFP expression in either the wild type or in animals carrying a gain-of-function (denoted as egl-43(gof)) mutation in their FOS-1-binding site upstream of the egl-43. PVD and PDE nuclei are outlined (white dashed circles). This schematic was created with BioRender.com. f, Quantification of EGL-43::GFP intensity shown in e (n = 14 for both conditions). g, Line scans of FLP-on ELKS-1 in the PDE axon in the wild type and animals carrying egl-43(gof). h, Quantification of line scans of animals shown in g (n = 10 for both conditions, P = 5.45276 × 10−7). i, Line scans of FLP-on RAB-3 in the PDE axon in the wild type and animals carrying egl-43(gof). j, Quantification of line scans of animals shown in i (n = 10 for both conditions, P = 2.18919 × 10−5). For all graphs, medians are represented in thick dashed lines and quartiles are represented in thin dashed lines. P values displayed in this figure were calculated using two-tailed unpaired Student’s t tests. Scale bar for all images = 10 μm.
