Fig. 7: EGL-43 regulates the expression of activity-dependent TFs and hardwired genetic pathways to promote presynaptic gene expression.
From: An activity-regulated transcriptional program directly drives synaptogenesis
a–l, Left: images of L4 animals carrying endogenously tagged GFP or mNG TFs and EGL-43::TagRFP-T::AID* treated with 0 mM (A(−)) or 4 mM auxin (A(+)) for 48 h. PVD and PDE neuronal nuclei are outlined (white dashed circles). Right: quantification of TF intensity in the images shown on the left. Classification of the following factors tested with sample sizes: activity-dependent TFs JUN-1, n = 11 (A−) and n = 11 (A+) (a); CRH-1, n = 13 (A−) and n = 13 (A+) (b); EGRH-1, n = 10 (A−) and n = 10 (A+) (c); MEF-2, n = 10 (A−) and n = 11 (A+) (d). Classification of the following dopaminergic terminal selectors/associated factors: CEH-43, n = 14 (A−), n = 21 (A+), P = 8.6174 × 10−6 (e); AST-1, n = 15 (A−), n = 15 (A+) (f); CEH-20, n = 15 (A−), n = 15 (A+), P = 3.84033 × 10−11 (PDE) (g); UNC-62, n = 12 (A−), n = 14 (A+), P = 1.15877 × 10−12 (PVD), P = 2.78114 × 10−8 (PDE) (h). CUT homeobox TFs are as follows: CEH-38, n = 12 (A−), n = 10 (A+) (i); CEH-41, n = 12 (A−), n = 9 (A+), P = 4.09353 × 10−9 (PVD) (j); CEH-44 n = 8 (A−), n = 10 (A+) (k); CEH-48, n = 12 (A−), n = 10 (A+) (l). m, Line scans of PDE axons from animals expressing endogenous FLP-on RAB-3. Mutations were generated in the promoter of rab-3 corresponding to the CUT, EGL-43 or both CUT and EGL-43 motifs. n, Quantification of the line scans shown in m (n = 15 for all genotypes). For all graphs, medians are represented in thick dashed lines, and quartiles are represented in thin dashed lines. P values were calculated using ordinary one-way ANOVA except for e and f, which were calculated using two-tailed unpaired Student’s t tests. Scale bar = 5 μm for images of nuclei; scale bar = 10 μm for line scans of PDE axons.
