Fig. 1: OLs abundantly express key amyloidogenic pathway genes as assessed by scRNA-seq and snRNA-seq.

a, UMAP visualization of cell types from a 6-month-old mouse brain snRNA-seq dataset⁹. b, Feature plots showcasing expression of key amyloidogenic genes (App, Bace1, Psen1 and Psen2) across all cell types in WT mouse brains. c, Expression level half violin plots of key amyloidogenic genes in neurons and OLs of mouse brains normalized by the SCTransform method, highlighting the similar expression of all genes between neurons and OLs. d, Expression level half violin plots of key amyloidogenic genes in neurons and OLs normalized by the SCTransform normalization method from additional mouse datasets^9,10,11. e, Mouse nervous tissue inputs for sequencing from each study are shown. f, UMAP visualization of cell types from a human brain snRNA-seq dataset¹². g, Feature plots showcasing expression of key amyloidogenic genes (APP, BACE1, PSEN1 and PSEN2) across all cell types in control human brains. h, Expression level half violin plots of key amyloidogenic genes in neurons and OLs of human brains with the SCTransform normalization method, highlighting the similar expression of these genes between neurons and OLs. i, Expression level half violin plots of key amyloidogenic genes in neurons and OLs normalized by the SCTransform normalization method from additional human datasets^13,14. j, Positive rate bar plots of APP processing genes in mouse and human nervous tissue inputs. k, Human nervous tissue inputs for sequencing from each study are shown. c,d,h,i, Half violins represent aggregated expression levels of respective genes from each cell type, and data points refer to individual expression levels from single cells or nuclei normalized by SCTransform. The results published here are based on data obtained from the Gene Expression Omnibus and the AD Knowledge Portal. mo, months; UMAP, uniform manifold approximation and projection.