Extended Data Fig. 7: Microgliosis is proportional to Aβ burden.

(a) Coronal sections of female control, OL-Bace1cKO;AD and ExN-Bace1cKO;AD mouse hemibrains stained for microglia (Iba1) and Aβ (6E10). (b,c) Closeup images of cortex and WM of control and cKO mice showing moderate and marked reductions of both Aβ deposits and microgliosis in OL-Bace1cKO;AD and ExN-Bace1cKO;AD samples, respectively. Inherent changes in microgliosis could thus be excluded as microglia only appear reactive to plaques and not in regions devoid of them. (d,e) Quantification of Aβ load and microgliosis between controls and cKOs (n = 5 per group) spanning different regions. Microgliosis was shown to be directly linked to plaque load. One-way ANOVA was performed with Tukey multiple comparison tests (P values indicated in graphs with significance highlighted in bold). Bars represent means with SEM and individual data points displayed.