Fig. 2: OLs contribute to Aβ burden primarily derived from ExNs in vivo.
a, Mouse breeding setup to investigate the OL-specific and ExN-specific contributions to Aβ burden. b–f, LSM data of plaque burden (Congo red) comparing 6-month-old OL-Bace1cKO;AD mice to age-matched and sex-matched littermate controls. g–k, LSM data of plaque burden (Congo red) comparing 6-month-old ExN-Bace1cKO;AD mice to age-matched and sex-matched littermate controls. b–k, Color-region allocation is as follows: white, isocortex; blue, hippocampus; yellow, alveus; pastel blue, inferior colliculus; pastel yellow, thalamus. b,g, LSM 3D visualization of control and cKO hemibrains. c,h, Brain region-segmented plaques of control and cKO hemibrains. d,i, LSM 2D single plane of control and cKO hemibrains. Arrowheads point to plaques with colors indicating specific regions. e,j, LSM 3D renders of representative cortical Aβ plaques of control and cKO hemibrains. f,k, Quantification of LSM data between controls (n = 5 per sex) and cKOs (n = 5 per sex). Normalization of cKO data points to sex-matched controls was performed. Circles represent controls, and triangles represent cKOs. Filled shapes represent male mice, and hollowed shapes represent female mice. For each parameter, unpaired, two-tailed Student’s t-test was performed (P values indicated in graphs with significance highlighted in bold) comparing cKOs to controls. Bars represent means with s.e.m., and individual data points are displayed. Raw data are available in Supplementary Tables 1 and 2.
