Extended Data Fig. 7: Hypomyelinated optic nerves in normally behaving GLUT1 icKO mice lack histological signs of pathology or gliosis.
From: Oligodendroglial fatty acid metabolism as a central nervous system energy reserve
a, Normal body weight of GLUT1 icKO mice at the age of 7 months (5 months post tamoxifen). (N = 6, CTR and icKO). b, Normal rotarod performance of GLUT1 icKO mice shown as the speed (rpm) at which the mice fall (same mice in a). c, Normal forelimb grip strength of GLUT1 icKO mice (same mice as in a). d, Quantification of the inner tongue size as a function of axon caliber, by plotting the axon diameter (ax) by the respective diameter of a circle defined by the inner surface of the compacted myelin sheath (il), analogous to g-ratios (same images in Fig. 4f). e, Tendency for smaller average inner tongue sizes in GLUT1 icKO mice. (same data as in d; CTR, N = n = 3; icKO, N = n = 4). f, Normal number (density) of optic nerve axons in GLUT1 icKo mice normalized to controls (same images from Fig. 4f). g, Normal axon size distribution in optic nerve from GLUT1 icKo mice (same images from Fig. 4f; CTR, N = n = 3; icKO mice, N = n = 4). h, Normal percentage of unmyelinated axons in GLUT1 icKO mice (same images from Fig. 4f). i, Normal percentage of axons showing morphological abnormalities by EM analysis (from images in Fig. 4f). j, Normal excitability of optic nerves from GLUT1 icKO mice, recorded with increasing current of stimulation. Calculated CAPA for each current was normalized to recorded CAPA at 0.75 mA (CTR, N = n = 9; icKO, N = n = 15). k, Normal optic nerve conduction velocity (NCV) of GLUT1 icKO mice, calculated by dividing latency of the second CAP peak to the length of the nerve (common data with j; CTR, N = n = 6; icKO, N = n = 12). l, Longitudinal sections of GLUT1 icKO optic nerves immunostained for Iba1 and counterstained with DAPI. m, Normal number of Iba1+ microglia in optic nerve from GLUT1 icKO mice, normalized to the number of DAPI+ nuclei in the same area (same images in l). CTR N = 3, n = 4, icKO N = n = 5. All animals (both genders) were analyzed 4-5 months post tamoxifen injections. All error bars: mean ± SEM, unpaired two-tailed t-test. CTR, Control.
