Extended Data Fig. 8: Optic nerves in GLUT1 icKO mice lack histological signs of changes in inflammatory/oligodendrocyte population or lipid droplets.
From: Oligodendroglial fatty acid metabolism as a central nervous system energy reserve
a, Longitudinal sections of GLUT1 icKO optic nerves immunostained for CC1 (orange) and counterstained with DAPI (blue). b, Oligodendrocytes population in optic nerve from GLUT1 icKO mice calculated by dividing the DAPI spots positive for CC1 to total number of DAPI+ nuclei (DAPI + CC1 + /DAPI + ) in the same area. Data points were normalized to the mean of CTR; N = 4 for both CTR and icKO. c, Longitudinal sections of GLUT1 icKO optic nerves immunostained for CC1 (orange), IL-33 (green) and counterstained with DAPI (blue). d-e, Bar graphs showing percentage of oligodendrocytes were positive for inflammatory marker, IL-33, (CC1 + IL-33 + ) divided to total number of oligodendrocytes (DAPI + CC1 + ) (d) and percentage of oligodendrocytes exhibiting high expression of inflammatory marker, IL-33 (e). (data points were normalized to the mean of CTR; N = 4 for both CTR and icKO). f, GLUT1 icKO optic nerve longitudinal sections were immunostained for Plin2 (red) and counterstained with DAPI (blue). Note lipid droplets are barely detectable in optic nerve cells (N = 3 for both control and icKO nerves). All animals (from both genders) were analyzed at the age of 6-7 months (4-5 months post tamoxifen). An unpaired two-tailed t-test was performed for comparing different groups. Error bars indicate mean ± SEM, and individual data points displayed. CTR, Control.
