Extended Data Fig. 6: Microglial and perivascular macrophage (PVM) interactions with pericytes are enhanced in AD mice.
a In vivo two-photon imaging in the barrel cortex of WT and AD x Iba1-eGFP x NG2-dsRed mice used to quantify the percentage of microglia/PVM somata <10 µm vs. >10 µm from the nearest pericyte soma. Yellow and light red triangles point at pericytes that are <10 and >10 µm from microglia/PVMs, respectively. b Microglia/PVMs are located closer to 1st-3rd order and >3rd order pericytes in AD mice (co-expressing NG2-dsRed either with Iba1-eGFP or CX3CR1-eGFP) than in WT mice. c CD206-labeled PVMs in the pia mater (top panel), along venules and arterioles (bottom left and right panel), and on capillaries of the 1st-2nd branch order off an arteriole (bottom middle and right panels). Blue triangles point at pericytes that are <10 µm from PVMs. Yellow and light red triangles point at pericytes that are <10 and >10 µm from P2Y12R-labeled microglia, respectively. d Mean number of PVMs per capillary segment decreases as the branch order from the arteriole increases (quantified from images as shown in c). e Percentage of 1st-3rd order pericytes closest to a CD206 or P2Y12R-labeled cell in fixed cortical tissue from WT and AD mice. f Distance of 1st-3rd order pericyte soma to P2Y12R or CD206-expressing soma quantified from images such as shown in e. g Fixed AD cortical tissue imaging of Alexa Fluor 647 conjugated NOX2 antibody (or 647-excited autofluorescence control) in microglia and PVMs. P values in panels a and upper bar graph of f are from unpaired t tests and in b and lower bar graph of f from Mann-Whitney tests. P-values are 2-tailed. Error bars are s.e.m.
