Extended Data Fig. 3: Macrophage/ microglia promote angiogenesis in the ventricular zone (VZ) of the ganglionic eminences.

(a) Schematic diagram showing three schedules of CSF1R inhibitor PLX5622 injection in pregnant mouse dams. (b, d, f) Transient inhibition of CSF1R leads to significant depletion of IBA1+ cells in the VZ of GE (b), VZ/SVZ of the pallium (d), and the CP (f). (c, e, g) Quantification of densities of IBA+ cells and IB4+ blood vessels in the VZ of GE (c), VZ/SVZ of the pallium (e), and the CP (g). Dashed lines indicate the regions in which quantifications are performed. Statistics in panels c, e, and g use two-tailed, unpaired Student’s t-test, data represent mean ± SEM. ns, not significant. n indicates the number of independent biological samples used for quantification.