Fig. 1: EM reconstructions of brainstem neurons.
From: Predicting modular functions and neural coding of behavior from a synaptic wiring diagram
a, Three-dimensional rendering of reconstructed neurons. The large green cell body in the foreground is the Mauthner neuron (Mcell); Ro, rostral; C, caudal; D, dorsal; V, ventral; L, lateral; M, medial. The inset (top left) shows the location of the unilateral EM volume (black box) relative to the olfactory bulb (OB), tectum (Te), hindbrain (HB) and spinal cord (SC). b–d, Automatic synapse detection and partner assignment. b, Raw EM image; scale bar, 750 nm. c, Postsynaptic densities (PSDs) identified by a convolutional network. d, Postsynaptic densities (red) overlaid onto the original raw image together with an exemplar presynaptic (blue) and postsynaptic (yellow) partnership identified by a second convolutional network. e, Sagittal view of the identified ABDM (green) and ABDI (magenta) neurons overlaid over representative EM planes; R, rhombomere. The asterisk (*) indicates the Mauthner cell soma. f, Coronal planes showing the locations of the ABDM (left) and ABDI (right) neurons at the planes indicated by dotted black lines in the sagittal view. Black boxes highlight nerve bundles from these populations. g, Representative ABDM and ABDI neurons with arrows indicating the axons. h, Reconstructions of large and small RS neurons and Ve2 neurons.
