Extended Data Fig. 1: Allen Brain Atlas-based search for potential top–down breathing circuits.
From: A top-down slow breathing circuit that alleviates negative affect in mice
a, Summary of the criteria used to search candidate circuits. Axonal projection images were searched through the Mouse Brain Connectivity Atlas (Allen Brain Institute). In situ hybridization (ISH) images were searched through the Mouse Brain ISH Data Atlas (Allen Brain Institute). b, Sample images showing the projections of labeled PnC neurons observed in the ventrolateral medulla (VLM). Left, the AAV injection site (PnC). Right, the observed terminals in the VLM. c, Sample image from a Vgat ISH experiment. d, Sample images showing the projections of PFC neurons observed in the PnC. Left image shows the AAV injection site (PFC, prefrontal cortex; covering the dACC and vACC) with the expression of eGFP. Middle and right (in segmentation view) images show the eGFP-labeled axon terminals in the PnC. PnC, pontine reticular nucleus; VLM, ventrolateral medulla; PFC, prefrontal cortex. Scale bars, 200 μm in b–d. These are representative data (b–d) available from the Allen Brain Atlas.
