Extended Data Fig. 6: Live STED confirms LatA and CytoD, but not blebbistatin or nocodazole, disrupt actin ring organization.
From: Membrane mechanics dictate axonal pearls-on-a-string morphology and function
a–g. Representative 2D STED images of live axon stained by SiR-actin under control (DMSO) or drug treatments with plotted intensity profile from region indicated by blue line and fast Fourier transform of intensity profile where the largest peak represents the most frequent distance between actin puncta. Histogram (black) and Gaussian distribution (red line) of actin puncta spacing from 50 axons in each condition from 2 independent cultures. a, DMSO control, Gaussian distribution values: avg. 245 nm S.D. 253 nm. b, LatA 30 min, Gaussian distribution values: avg. 352 nm S.D. 351 nm. c, DMSO control, Gaussian distribution values: avg. 235 nm S.D. 184 nm. d, LatA 1 hr, Gaussian distribution values: avg. 288 nm S.D. 265 nm. e, CytoD 1 hr, Gaussian distribution values: avg. 772 nm S.D. 482 nm. f, Blebbistatin 1 hr, Gaussian distribution values: avg. 152 nm S.D. 43 nm. g, Nocodazole 1 hr, Gaussian distribution values: avg. 174 nm S.D. 69 nm.
