Extended Data Fig. 8: Axonal pearling changes with stimulation even after cholesterol manipulation.
From: Membrane mechanics dictate axonal pearls-on-a-string morphology and function
a. Additional example micrographs showing axon morphology from neurons unstimulated or stimulated with 3 × 100 pulses at 100 Hz (high-frequency stimulation, HFS) and high-pressure frozen at 5 min and 30 min after stimulation. Scale bar: 200 nm. b. Super Plots of Fig. 5b, showing experimental variability. N = 3 independent cultures except for control 15’ post stim which was N = 2. n = 100 axons each. c. Super Plots of Fig. 5c, showing experimental variability. N = 3 independent cultures. n = 100 axons each. In all super plots median and 95% confidence intervals are shown. N = 3 independent cultures and n = 100 axons each. Each color represents one replicate. Each dot is one axon. All data analyzed using Kruskal-Wallis test, followed by Dunn’s multiple comparison test.
