Extended Data Fig. 3: Manipulation of osmolarity causes changes in axonal pearling.
From: Membrane mechanics dictate axonal pearls-on-a-string morphology and function
a. Additional example micrographs of axons high-pressure frozen at indicated osmotic conditions. Scale bar: 200 nm. b. Super Plots of Fig. 2f, showing experimental variability. c. Example micrographs of axons high-pressure frozen at indicated osmotic conditions. Mannitol was used to adjust the osmolarity. Scale bar: 200 nm. d. Super Plots of Extended Data Fig. 2e, showing the dimensions of NSVs at indicated osmotic conditions. e. Plot showing the dimensions of NSVs at indicated osmotic conditions. Mean and SEM are plotted. N = 3 independent cultures, n = 100 axons each. In all super plots median and 95% confidence intervals are shown. N = 3 independent cultures and n = 100 axons each. Each color represents one replicate. Each dot is one axon. All data analyzed using Kruskal-Wallis test, followed by Dunn’s multiple comparison test.
