Extended Data Fig. 4: Manipulation of membrane cholesterol causes changes in axonal pearling.
From: Membrane mechanics dictate axonal pearls-on-a-string morphology and function
a. NeonGreen-ALOD4 staining of cultured mouse hippocampal neurons treated with sham (control) or 5 mM MßCD for 30 min. Scale bar = 5 µm. b. Plot showing the normalized intensity of NeonGreen-ALOD4. Signals are normalized by the area of each region-of-interest. Control: 2773 ± 234 A.U., MβCD: 86 ± 13 A.U., median and 95% confidence intervals are plotted, Mann-Whitney U test (two-sided), N = 3 independent cultures, n = 127 for control, n = 131 for MβCD. A.U. arbitrary unit. c. Additional example micrographs of axons high-pressure frozen after MßCD treatment. Scale bar: 200 nm. d. Super Plots of Fig. 2h, showing variability. N = 3 independent cultures and n = 100 axons each. Median and 95% confidence intervals are plotted, Kruskal-Wallis test, followed by Dunn’s multiple comparison test. Each color represents one replicate. Each dot is one axon.
