Table 1 Average dimensions and s.e.m. of axon NSVs and connectors
From: Membrane mechanics dictate axonal pearls-on-a-string morphology and function
Sample name | Sample type | Treatment | NSV length (nm) | NSV width (nm) | Connector length (nm) | Connector width (nm) | |
|---|---|---|---|---|---|---|---|
Organotypic | Organotypic | 326 ± 7 | 177 ± 3 | 252 ± 10 | 52 ± 2 | ||
Acute | Acute slice | 357 ± 10 | 204 ± 6 | 380 ± 15 | 58 ± 1 | ||
Dissociated | 2D | 600 ± 9 | 311 ± 5 | 552 ± 14 | 127 ± 3 | ||
Chemical treatments | 0.2% DMSO | 2D | DMSO, 30 min | 630 ± 13 | 318 ± 5 | 603 ± 18 | 136 ± 3 |
2D | DMSO, 1 h | 632 ± 18 | 323 ± 7 | 584 ± 20 | 153 ± 5 | ||
0.1% DMSO | 2D | DMSO, 1 h | 602 ± 12 | 351 ± 7 | 518 ± 19 | 115 ± 3 | |
LatA | 2D | LatA, 30 min | 625 ± 11 | 323 ± 5 | 574 ± 20 | 136 ± 4 | |
2D | LatA, 1 h | 584 ± 21 | 326 ± 11 | 588 ± 18 | 123 ± 6 | ||
CytoD | 2D | CytoD, 1 h | 640 ± 17 | 342 ± 9 | 611 ± 19 | 134 ± 4 | |
Blebbistatin | 2D | Blebbistatin, 1 h | 590 ± 11 | 295 ± 4 | 431 ± 12 | 215 ± 5 | |
Nocodazole | 2D | Nocodazole, 1 h | 587 ± 10 | 297 ± 3 | 446 ± 13 | 195 ± 6 | |
MβCD | 2D | MβCD 5 mM | 550 ± 17 | 312 ± 8 | 540 ± 20 | 116 ± 4 | |
Control | 2D | No treatment | 640 ± 17 | 310 ± 7 | 490 ± 15 | 136 ± 4 | |
Sptbn1 KD | 2D | Scb | 728 ± 15 | 363 ± 6 | 633 ± 19 | 178 ± 6 | |
2D | Sptbn1 KD | 651 ± 14 | 335 ± 6 | 585 ± 19 | 138 ± 5 | ||
Osmolarity | 2D | 300 mOsm | 480 ± 9 | 275 ± 5 | 410 ± 15 | 111 ± 2 | |
2D | 600 mOsm | 370 ± 8 | 190 ± 4 | 430 ± 16 | 106 ± 3 | ||
2D | 150 mOsm | 610 ± 13 | 390 ± 8 | 661 ± 21 | 107 ± 3 | ||
2D | 300 mOsm | 510 ± 10 | 250 ± 4 | 490 ± 19 | 114 ± 3 | ||
2D | 280 mOsm | 565 ± 10 | 268 ± 4 | 540 ± 16 | 125 ± 3 | ||
2D | 400 mOsm | 472 ± 8 | 228 ± 3 | 560 ± 13 | 118 ± 3 | ||
Stimulation | No stim | 2D | 449 ± 12 | 232 ± 5 | 462 ± 15 | 118 ± 3 | |
5 min | 2D | 541 ± 15 | 262 ± 6 | 512 ± 18 | 135 ± 4 | ||
30 min | 2D | 526 ± 14 | 287 ± 7 | 510 ± 18 | 118 ± 4 | ||
MβCD no stim | 2D | MβCD 5 mM | 405 ± 10 | 242 ± 6 | 421 ± 15 | 97 ± 3 | |
MβCD 5 min | 2D | MβCD 5 mM | 477 ± 14 | 293 ± 9 | 463 ± 15 | 111 ± 3 | |
MβCD 30 min | 2D | MβCD 5 mM | 448 ± 13 | 278 ± 7 | 426 ± 16 | 108 ± 3 |
