Extended Data Fig. 8: Details about tagging first-order somatosensory neurons and axonal bouton imaging.
From: Emergence of a brainstem somatosensory tonotopic map for substrate vibration
a, Expression of GCaMP6s in the first-order somatosensory neurons in DRG was achieved by viral injection at the dorsal horn of lumbar enlargement. b, The two photon imaging of the brainstem (top left) and a whole-mount skin sample from the toe tip (top right) showed the two extreme terminals of the first-order somatosensory neurons. Bottom, three example fields of view at the DRG level, showing the labelling of the cell bodies. c, Four example images of dissected spinal cord revealed the heterogeneity of the viral injection and expression. d, Top, the percentage of different groups of DRG neurons defined by three mechanosensitive end-organs-specific stimulation. Bottom, the percentage of seven classified groups of axonal boutons in DCN defined by three mechanosensitive end-organs-specific stimulation. Those multimodal boutons in DCN are likely from the indirect pathway through the spinal cord dorsal horn.
