Extended Data Fig. 9: Histology and illustration of imaging in the complete somatosensory pathway for touch.
From: Emergence of a brainstem somatosensory tonotopic map for substrate vibration
a, Left, schematic representation of the ascending pathway of the somatosensory system. Right, the example photos of each location where the imaging windows were installed. b-c, The anatomical-defined receptive fields of lumbar DRG cells were mapped by injecting fluorescent lipophilic dye DiI, DiO and DiD into different locations of the hindlimb. d, Example photos of five DCN windows. White dashed lines marked the right gracile nucleus. e, Overlaying of the gracile nuclei across 24 animals yielded a template for alignment. f, Three tangential serial sections (100 ÎĽm apart) of the DCN showing the viral expression covered the complete right gracile nucleus. White dashed lines marked the right gracile nucleus. g, Four example post hoc histological verification of the GRIN lens implantation above the thalamus (VPL) after the viral expression of GCaMP7b. h, Example epifluorescence imaging of the brain showing the GCaMP expression at the injection site at S1 and the projections at the contralateral DCN.
