Extended Data Fig. 4: Comparing different generations of GCaMP imaging and electrophysiology.
From: Emergence of a brainstem somatosensory tonotopic map for substrate vibration
a, The example tuning curve of a DCN neuron across different vibration frequencies at the vibration amplitudes of 3 µm: grey dots, individual stimulus responses; grey trace, mean values; black trace, polynomial curve fit; vertical green dashed line, preferred frequencies; red horizontal lines, the half-width at the half-maximum of the curve. b, Left, the distribution of the tuning preference from the DCN imaging experiments performed with different generations of calcium indicator, GCaMP. Right, the distribution of the tuning width measured in the different imaging experiments. c, Left, the distribution of the tuning preference measured from the calcium imaging at the DRG (top) or electrophysiology recording at the nerve fibers (bottom). Right, the distribution of the tuning width measured from the calcium imaging (top) or electrophysiology experiments (bottom). d, Left, the distribution of tuning preferences from individual DCN neurons measured using calcium imaging from the population of thalamic projection neurons (top) or inferior colliculus projection neurons (bottom). Right, the distribution of the tuning width from the population of thalamic projection neurons (top) or inferior colliculus projection neurons (bottom). No significant difference was found in any comparison within DCN or DRG with the Kolmogorov-Smirnov test, two-sided. e, Left, example fields-of-view of thalamic projection neurons in DCN (top) and each neuron's preference for location and frequency colour-coded (bottom). Right, the relationship of horizontal distance between neurons in a pair and their difference in location (top) and frequency (bottom) is calculated (*P < 0.01, permutation test). The slope of the linear fit (SLF, italic) of the first 5 data points from the original data (black line) is shown. Error bars represent S.E.M. f, Left, example fields-of-view of inferior collicular projection neurons in DCN (top) and each neuron's preference for location and frequency colour-coded (bottom). Right, the relationship of horizontal distance between neurons in a pair and their difference in location (top) and frequency (bottom) is calculated (*P < 0.01, permutation test). The slope of the linear fit (SLF, italic) of the first 5 data points from the original data (black line) is shown. Error bars represent S.E.M. n/N = number of neurons and mice.
