Fig. 1: Generation of humanized mutant MAPT KI mice with BEs. | Nature Neuroscience

Fig. 1: Generation of humanized mutant MAPT KI mice with BEs.

From: In vivo hyperphosphorylation of tau is associated with synaptic loss and behavioral abnormalities in the absence of tau seeds

Fig. 1

a, Schematic image of BE-mediated genome editing of the MAPT gene. b, Schematic illustration of BE-mediated genome editing in MAPT KI mouse zygotes by microinjection. c, Sanger sequencing results determined MAPTInt10+3 (upper panel) and MAPTS305N;Int10+3 (lower panel) KI mice. Mutation loci are indicated with arrowheads in black and substituted amino acids highlighted in red. d, Regional, genetic and annotated information regarding off-target sites identified in MAPTInt10+3 and MAPTS305N;Int10+3 KI mice. Alt., alternative; chr, chromosome; ref., reference. Schematic in a created using BioRender.com.

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