Extended Data Fig. 3: RT-PCR, LC-MS/MS analysis and immunostaining of tau in MAPT KI, MAPT^Int10+3 KI and MAPT^{S305N; Int10+3} KI mice.

a, The relative expression of 3 R and 4 R tau were calculated by semi-quantitative RT-PCR using the brain from Mapt KO, MAPT KI, MAPT^Int10+3 KI, and MAPT^{S305N; Int10+3} KI, and PS19 (n = 3 for each group). b, Amount of tau peptides based on the isoform resulting from alternative splicing by LC-MS/MS analysis in the brains of 12-15 month-old MAPT KI (n = 6; sex-matched), MAPT^Int10+3 KI (n = 5; n = 3 female and n = 2 males), and MAPT^{S305N; Int10+3} KI mice(n = 9; n = 4 females and n = 5 male). 4R-tau (299-317 aa) in MAPT^{S305N; Int10+3} KI line was not detected because serine (S) at position 305 was converted to asparagine (N). c-e, Immunostaining of 3 R and 4 R tau in the brains of 3-month-old MAPT KO, MAPT KI, MAPT^Int10+3 KI, and MAPT^{S305N; Int10+3} KI mice (n = 4 for each group, n = 2 females and n = 2 males). Scale bar represents 1 mm. Data in (b), (d), and (e) represent the mean ± SEM. (two-way ANOVA followed by Tukey’s multiple comparison test).

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