Extended Data Fig. 2: Localization of GFP-tagged NOMPC variants in Drosophila JO neurons.
From: NOMPC ion channel hinge forms a gating spring that initiates mechanosensation
GFP signals are enhanced with an anti-GFP antibody (cyan), and JO neurons are counterstained with anti-horseradish peroxidase (HRP), which recognizes sugar residues of glycoproteins that are secreted by JO neurons in two bands (h, see inset upper right). The proximal band (arrowhead) demarks the junction between the cilium (c) and the dendritic inner segment (d), and the distal band demarks the ciliary dilation (“cd”) that separates cilium tip and base regions. s: Cell soma. Anti-GFP staining shows that NOMPC::GFP, AR + AR-NOMPC::GFP, and LH + LH-NOMPC::GFP localize to the cilium tip regions, same as native NOMPC (lower panel, stained with the anti-NOMPC antibody αNOMPC-EC16. Scale bars: 20 µm. n = 3 flies/strain.
