Extended Data Fig. 3: Elasticities of LH and AR domains.
From: NOMPC ion channel hinge forms a gating spring that initiates mechanosensation
We simulated two systems separately to calculate the spring constants of the LH+5ARs (a, TMD + LH+5ARs) and the ARs (b, LH+26ARs). Only one subunit is shown in ribbon for clarity, with the transmembrane domain (TMD), the TRP helix, the LH domain and the ARs in gray, cyan, orange and magenta. The ARs on which forces were applied are labelled by green lines. For panel b, we also show the structure of a subunit, which is coloured based on the root-mean-square-fluctuation (RMSF) of the Cα atom of each residue (simulations with a force of 30 kJ/(mol nm) applied to AR4 are used as an example). For additional information, see Methods and Supplementary Table S2.
