Extended Data Fig. 8: Recurrent inhibition gates spiking activity of 5-HT neurons.
From: Nonlinear recurrent inhibition through facilitating serotonin release in the raphe
a, Schematic of experimental design. Left, AAV-DIO-ChETA-YFP is injected into the DRN at a 2:1 ratio with Mannitol. Right, whole-cell recordings from ChETA-negative 5-HT neurons. b, Recurrent 5HT1AR-mediated current in ChETA-negative 5-HT neuron. c, Spiking of a 5-HT neuron in response to current injection. Below, raster plot showing the same neuron’s activity across trials. d, Spiking of a 5-HT neuron in response to current injection and 20 Hz optical activation of ChETA-positive 5-HT neurons. Below, raster plot showing the same neuron’s activity across trials. e, Spiking of a 5-HT neuron in response to current injection in the presence of WAY100-635. Below, raster plot showing the same neuron’s activity across trials. f, Spiking of a 5-HT neuron in response to current injection and 20 Hz optical activation of ChETA-positive 5-HT neurons, in the presence of WAY100-635. Below, raster plot showing the same neuron’s activity across trials. g, Smoothed firing rate (normalized) over time for pre- and post-WAY recordings with LED stimulation (mean +- SE, n = 3 neurons). h, Cumulative spike fraction in pre- and post-WAY recordings with LED stimulation (n = 3 neurons, two-sided Kolmogorov-Smirnov test: p = 7.4*10−15). i, Smoothed firing rate (normalized) over time for pre- and post-WAY recordings without LED stimulation (mean +- SE, n = 3 neurons). j, Cumulative spike fraction in pre- and post-WAY recordings with LED stimulation (3 neurons, two-sided Kolmogorov-Smirnov test: p = 0.85).
